method for enamel separation and purification in large quantity by a modified bromoform floatation method

An improved bromoform floatation method which permits the separation of relatively large amounts of enamel from ground tooth crowns, in a short time has been developed. Clean tooth crowns were dried, crushed and centrifuged at 2000 rpm for 2 minutes repeated two, times in bromoform. Enamel deposits were separated. They were dull grey in color. Two washes with acetone plus three washes with water succeeded in minimizing the bromide content of the separated enamel to be comparable with the bromide content of mechanically separated enamel. After purification the enamel became slightly off-white in color. The yield of purified separated enamel was 34 percent from the powdered crowns